LOCUS 9587 bp ds-DNA circular 07-MAY-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 1230..1247 /ApEinfo_label=N-terminal hexahistidine tag /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 CDS 1278..1991 /ApEinfo_label=GFP(S2G/S65C) /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 CDS 1251..1274 /ApEinfo_label=FLAG epitope(1) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 gene 7353..9587 /ApEinfo_label=LEU2 /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 terminator 2038..3029 /ApEinfo_label=nmt1 terminator /ApEinfo_fwdcolor=#7f7f7f /ApEinfo_revcolor=#7f7f7f rep_origin 3042..4234 /ApEinfo_label=ars1 /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=#8000ff promoter 36..1201 /ApEinfo_label=nmt1* promoter /ApEinfo_fwdcolor=#ff6fcf /ApEinfo_revcolor=#ff6fcf ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGATCGACTC TAGAGGATCA GAAAATTATC GCCATAAAAG 61 ACAGAATAAG TCATCAGCGG TTGTTTCATT TCCTATATTT TTTTTTTATT TTTTTATTTT 121 TTAATAAGGG AAAATTTAAC GTCTAAGGAT ACAGAAGATT GTTAGCACAT TAAAGTAATA 181 AAGGCTTAAG TAGTAAGTGC CTTAGCATGT TATTGTATTT CAAAGGACAT AATCTAAAAT 241 AATAACAATA TCATTTCTCA CAAGTTATTC AATTTTCTTT TTTTTTTCTA ATAATATCAA 301 GAATGTATTA TTTGTTTGAC ATAAGTCAAC TAATTTATTT AATATGCTGG ATTAATCTTG 361 CAGACATGTA AATTAACAAG TTTTAGTCAA ATAACGTTGA AGTTTCAATG AACTCAAATA 421 ATTTCTCTTT TTTTTTATAT AACTCTAATC TGATTTATAT TTTCCGCAGG GATCAACTGA 481 AGTTATGACA TTTGGATTGG ATCACTTATA ACCTTGGTCG CCAAATAATA CAAAAATCAG 541 CGTTATAAAA CAAAGAAGGT TTTTGTTAAG AAATTAATCC TCTTTCTTGA TAAGAAAGTT 601 GAACCGAAAT TGCAGATACT GATATATGAA AATAATACCC ACAATTTTGG GAATAGCGCA 661 AGCCTCAATT TAAACAATAG GTGAGGACAC ATGATAATGA CCTCAATGAT TGTTAGAAGA 721 AAAGAGCCTC ATTACAAAAT CGAAAAATGA ATGGTTGGGT ACAAGTTTCC AAAACATGGT 781 AAAGTGGACT TTGCGTATGA GACGTAAATA GAAAAAAACA CTTGTTATAT GTTTTCTAGA 841 ATTATTGTTG TCTCTTTATG GTTGGATGAT GCAAAATAGT AATTTCGGTT AGTTGCTGTA 901 AAACACCACG AGACAAATAG ATATGGATAT TTATTAAATC AGGAAAAACG TAACTCTCGG 961 CTACTGGATG GTTCAGTCAC CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG 1021 CTTAAAGGAA TCCGATTGTC ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA 1081 CCTGTTAATC GAAACATTGA AGAATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA 1141 TAAGTTGAAT TAATTATTTC AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA 1201 AGCTAGCGTC GACAGGCCTG GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG 1261 ATGATGACGA TAAACCCATG GGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC 1321 TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG 1381 GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG 1441 TTCCGTGGCC AACACTTGTC ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC 1501 CAGATCACAT GAAACGGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG 1561 AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG 1621 AAGGTGATAC CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA 1681 ACATTCTTGG ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG 1741 ACAAACAAAA GAATGGAATC AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA 1801 GCGTTCAACT AGCAGACCAT TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT 1861 TACCAGACAA CCATTACCTG TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA 1921 GAGACCACAT GGTCCTTCTT GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG 1981 AACTATACAA AGATATCATG AGATCTCGAG CCCGGGCGGC CGCTAACTAA CTAAGGGTAA 2041 AAGGAATGTC TCCCTTGCCA GTACTGCTAG GGTTTTTCTT TCAAACTATG GAAGCCCATT 2101 CAAGCTGCAT ATTACGATTT TGTTTTTCGC TTTTAGAAAG TGGTTTAGAT GAGATAATAG 2161 AAAAATTCTT GATCTCCGAC AACGAGTACT TTTATTTTTT TTGCTAATCA CTTTACTCAA 2221 TATTAGCTCG AAATCGTAGA AACGTAGACG GGTGCGGGAT ACCGAGTGGT GTAGTTAAGA 2281 ATTTTTATAA ACCACGTGGC CCAAAAATAT GAACCCAAAA CGTTTATACA TGAGTATACT 2341 TTAAGAAGGC TATACCCCTT CGTGTTAGAT GTAGTTTTAG CTACCCAACC CGAGTCTATG 2401 AGCTTGACTT CAGATGTAGA AGGCATTAAA TCGTTTTGAA TATTAATTAA AAAACGATGA 2461 AAATTAAATA TTTAAAAGCA ATCATACGCT GAAAATTTAG TGCTGTGGCT AATCCTTCAA 2521 CATGGAAATG CCATAAAAGT GACTTTGACA AAAAAAAAAG TATATACAGG TAGTAAACTC 2581 ATCTACTTCA TTGACTTTGT TTACAGCATG TGGAAGGAGG AATATTTATT GCTAAATCGT 2641 AGTTTAACAT TCAATAAGTA ATACTATTGA AATTCGACAA GATTGGCCGC ATGGATGAAA 2701 AAGAGGCATT TTGCTTTGGG AGAATTAGTT CAAATTAGAA CTGAAAAAAA AAACTTTACG 2761 AGGCAAAAAT GTCGGATTGA GATCGTAAAA GTTCGCTCGT CGTCTTTTGC TTTGTGATTG 2821 TTTTCATGGA TACATCTTGC TGGATATTTA AATTTTAGTA CTATGTATAA GATATTCTAT 2881 AAATGTTTTA TCACCCAAAC CTGTTAGCGC CTTCTTAATT CTATTCAATC TGGCTTTTGC 2941 TCTGAGACTA CTTCTTGGAC TTTCACTACT TGTTAGTTAT ACGGAATTTG TGTAATTAGA 3001 AGTGAAATAA TCCTTTCTAT TAGTAATGCG AGCTCGAATT CGAGTCTAAC TCCTTAACCA 3061 CTCGGACATA GTGACTTATC TGACACTCAT TGTAAATTAA TATATATATA GGCATTTTGT 3121 TTAGTTAAAG GTACTTAAGT AATTAGTATA AACGAACCAA TTTTATAATC AGGAAGTTAA 3181 GTGAATGGTA GCACATGTCG TAAAAATTGT GAATTTTTAT TGAATAATAT TTTAAATACA 3241 AGCCTTTCTA GACTAGGTAT ACTCATAAAC ATATATGAGC AAAAGGATAG AGGAGATTAC 3301 ATTGCATCTT CTACAAATTT ATTTATTGCC CTTTACTGAA AAATTAAATA ATGAGTACTA 3361 AATGATAAAA AGCGCTCAGT ACAAGAAAGA TTGCAAAAAT ATTGCATTCT TCATGAATTA 3421 AAGTTGCATA TAAGGCATAT TGAAAGTAAT AGTACTAAAA CAGCAGTTAG CGAAAATTAA 3481 TAGAATTATA TTCGCAAGAC AATTGTGACA TTAAATTAAA AAATTGTAGA ATTTTTACTA 3541 TCCTCTTTAA CGCCATGAGC CTTTATAAAA AGGTTAAATT AGTTTTAACA TTCTTTTTTT 3601 GAGTAAGAGT TTACAATTTA TCAAAACCTG TGTTATTATA TTCATTAGTT TCAATTTATT 3661 AGCATCTAGA GAAAAATCAA TTGGCAGTTA CATTGTTGGA ATTTATGAAG AAAAAGAATC 3721 TACAACGGAG AATAAGTTGC TGATCGCTTT CCCTAAAATT GTATATTTTG CTGAGCTTAT 3781 TTTGACATTT CGTTGAAGTT TTCTCTAATT CGCATTCATT TTAAGTAAAA CAATGAGAAA 3841 TAAAATTACA AAAAATACAA ATTAAAATAC AATTTTTAGC TATAATTATA GACGATGCCC 3901 TTGTATCCCA TTCTGTCTCG CTTGCCCCTA CTTTTTATCT TTTATATACC ATAATGAACG 3961 CTGCCGCTAC TAACCATACC CCGATTTTAC ATTTCGGACT CCCAAGGACG TACAAAATAG 4021 AAAACTATAG AAAAAAATAA TCAGAAAATA GCATGTCATC TCTTTGTAAA ACGCGTTTGC 4081 AAGAAGAAAG GAAACAATGG AGAAGAGATC ATCCATTTGT ATGTAAATTT TAGTAAACTT 4141 GAAGAAATCA CTAACAACTT CTCTTACTTA GGGATTCTAT GCAAAACCTT GTAAATCATC 4201 TGATGGAGGA CTCGATTTAA TGAATTGGAA GGTTGGAATT CACTGGCCGT CGTTTTACAA 4261 CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT 4321 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC 4381 AGCCTGAATG GCGAATGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT 4441 TCACACCGCA TACGTCAAAG CAACCATAGT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 4501 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCTTA GCGCCCGCTC 4561 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 4621 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 4681 TTGATTTGGG TGATGGTTCA CGTAGTGGGA CATCGCCCTG ATAGACGGTT TTTCGCCCTT 4741 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 4801 ACTCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GTCTATTGGT 4861 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA 4921 CAATTTTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC 4981 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT 5041 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC 5101 CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA 5161 TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 5221 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 5281 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC 5341 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 5401 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA 5461 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 5521 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 5581 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC 5641 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 5701 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT 5761 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 5821 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 5881 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG 5941 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 6001 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG 6061 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 6121 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 6181 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 6241 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT 6301 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 6361 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 6421 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 6481 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 6541 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 6601 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 6661 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 6721 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 6781 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 6841 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 6901 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 6961 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG 7021 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG 7081 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC 7141 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG 7201 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC 7261 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG 7321 GAAACAGCTA TGACCATGAT TACGCCAAGC TTGTCGATCG ACTACGTCGT TAAGGCCGTT 7381 TCTGACAGAG TAAAATTCTT GAGGGAACTT TCACCATTAT GGGAAATGGT TCAAGAAGGT 7441 ATTGACTTAA ACTCCATCAA ATGGTCAGGT CATTGAGTGT TTTTTATTTG TTGTATTTTT 7501 TTTTTTTTAG AGAAAATCCT CCAATATCAA ATTAGGAATC GTAGTTTCAT GATTTTCTGT 7561 TACACCTAAC TTTTTGTGTG GTGCCCTCCT CCTTGTCAAT ATTAATGTTA AAGTGCAATT 7621 CTTTTTCCTT ATCACGTTGA GCCATTAGTA TCAATTTGCT TACCTGTATT CCTTTACTAT 7681 CCTCCTTTTT CTCCTTCTTG ATAAATGTAT GTAGATTGCG TATATAGTTT CGTCTACCCT 7741 ATGAACATAT TCCATTTTGT AATTTCGTGT CGTTTCTATT ATGAATTTCA TTTATAAAGT 7801 TTATGTACAA ATATCATAAA AAAAGAGAAT CTTTTTAAGC AAGGATTTTC TTAACTTCTT 7861 CGGCGACAGC ATCACCGACT TCGGTGGTAC TGTTGGAACC ACCTAAATCA CCAGTTCTGA 7921 TACCTGCATC CAAAACCTTT TTAACTGCAT CTTCAATGGC CTTACCTTCT TCAGGCAAGT 7981 TCAATGACAA TTTCAACATC ATTGCAGCAG ACAAGATAGT GGCGATAGGG TCAACCTTAT 8041 TCTTTGGCAA ATCTGGAGCA GAACCGTGGC ATGGTTCGTA CAAACCAAAT GCGGTGTTCT 8101 TGTCTGGCAA AGAGGCCAAG GACGCAGATG GCAACAAACC CAAGGAACCT GGGATAACGG 8161 AGGCTTCATC GGAGATGACA TCACCAAACA TGTTGCTGGT GATTATAATA CCATTTAGGT 8221 GGGTTGGGTT CTTAACTAGG ATCATGGCGG CAGAATCAAT CAATTGATGT TGAACCTTCA 8281 ATGTAGGGAA TTCGTTCTTG ATGGTTTCCT CCACAGTTTT TCTCCATAAT CTTGAAGAGG 8341 CCAAAACATT AGCTTTATCC AAGGACCAAA TAGGCAATGG TGGCTCATGT TGTAGGGCCA 8401 TGAAAGCGGC CATTCTTGTG ATTCTTTGCA CTTCTGGAAC GGTGTATTGT TCACTATCCC 8461 AAGCGACACC ATCACCATCG TCTTCCTTTC TCTTACCAAA GTAAATACCT CCCACTAATT 8521 CTCTGACAAC AACGAAGTCA GTACCTTTAG CAAATTGTGG CTTGATTGGA GATAAGTCTA 8581 AAAGAGAGTC GGATGCAAAG TTACATGGTC TTAAGTTGGC GTACAATTGA AGTTCTTTAC 8641 GGATTTTTAG TAAACCTTGT TCAGGTCTAA CACTACCGGT ACCCCATTTA GGACCACCCA 8701 CAGCACCTAA CAAAACGGCA TCAACCTTCT TGGAGGCTTC CAGCGCCTCA TCTGGAAGTG 8761 GGACACCTGT AGCATCGATA GCAGCACCAC CAATTAAATG ATTTTCGAAA TCGAACTTGA 8821 CATTGGAACG AACATCAGAA ATAGCTTTAA GAACCTTAAT GGCTTCGGCT GTGATTTCTT 8881 GACCAACGTG GTCACCTGGC AAAACGACGA TCTTCTTAGG GGCAGACATA GGGGCAGACA 8941 TTAGAATGGT ATATCCTTGA AATATATATA TATATTGCTG AAATGTAAAA GGTAAGAAAA 9001 GTTAGAAAGT AAGACGATTG CTAACCACCT ATTGGAAAAA ACAATAGGTC CTTAAATAAT 9061 ATTGTCAACT TCAAGTATTG TGATGCAAGC ATTTAGTCAT GAACGCTTCT CTATTCTATA 9121 TGAAAAGCCG GTTCCGGCCT CTCACCTTTC CTTTTTCTCC CAATTTTTCA GTTGAAAAAG 9181 GTATATGCGT CAGGCGACCT CTGAAATTAA CAAAAAATTT CCAGTCATCG AATTTGATTC 9241 TGTGCGATAG CGCCCCTGTG TGTTCTCGTT ATGTTGAGGA AAAAAATAAT GGTTGCTAAG 9301 AGATTCGAAC TCTTGCATCT TACGATACCT GAGTATTCCC ACAGTTAACT GCGGTCAAGA 9361 TATTTCTTGA ATCAGGCGCC TTAGACCGCT CGGCCAAACA ACCAATTACT TGTTGAGAAA 9421 TAGAGTATAA TTATCCTATA AATATAACGT TTTTGAACAC ACATGAACAA GGAAGTACAG 9481 GACAATTGAT TTTGAAGAGA ATGTGGATTT TGATGTAATT GTTGGGATTC CATTTTTAAT 9541 AAGGCAATAA TATTAGGTAT GTAGATATAC TAGAAGTTCT CCTCGAC //