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To download sequence files, please right-click the click button and choose "save" or "download".
All files are created using a free software "ApE".
Although you can open these files in a simple text format, we recommend to use ApE.
Attention!
When you use a series of vectors for chromosomal integration, please note that not all the transformants are proper integrants.
According to the previous paper [Genetics. 1994;136(3):849-56.], this phenomenon occurs due to gene conversion.
Although it was reported that gene conversion occurs in nearly 30% of the transformants, we estimate that this frequency is less than 10% in our pDUAL2 systems.
But we recommend to check expression of the tagged proteins before you use in your experiment.
It is enough to check two transformants in most cases.
| pDUAL2 series (Gateway-incompatible) |
|
Plasmid name
|
Backbone
|
Tag
|
Tag position
|
Promoter
|
Multicopy marker
|
Integration marker
|
Sequence
|
| pDUAL2 |
(pUC119)
|
-
|
-
|
-
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFF1 |
pDUAL2
|
His6-FLAG2
|
5'
|
nmt1
|
ura4+
|
ura4+, leu1-
|
 |
| pDUAL2-HFF21 |
pDUAL2
|
His6-FLAG2
|
5'
|
tub1
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFF41 |
pDUAL2
|
His6-FLAG2
|
5'
|
nmt1*
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFF61 |
pDUAL2
|
His6-FLAG2
|
5'
|
ef1a-c
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFF81 |
pDUAL2
|
His6-FLAG2
|
5'
|
nmt1**
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFG1 |
pDUAL2
|
His6-FLAG-GFP(S65C)
|
5'
|
nmt1
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFG41 |
pDUAL2
|
His6-FLAG-GFP(S65C)
|
5'
|
nmt1*
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-HFG81 |
pDUAL2
|
His6-FLAG-GFP(S65C)
|
5'
|
nmt1**
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-FFH1 |
pDUAL2
|
FLAG2-His6
|
3'
|
nmt1
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-FFH21 |
pDUAL2
|
FLAG2-His6
|
3'
|
tub1
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-FFH41 |
pDUAL2
|
FLAG2-His6
|
3'
|
nmt1*
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-FFH61 |
pDUAL2
|
FLAG2-His6
|
3'
|
ef1a-c
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-FFH81 |
pDUAL2
|
FLAG2-His6
|
3'
|
nmt1**
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-GFH1 |
pDUAL2
|
GFP(S65C)-FLAG-His6
|
3'
|
nmt1
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-GFH41 |
pDUAL2
|
GFP(S65C)-FLAG-His6
|
3'
|
nmt1*
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-GFH81 |
pDUAL2
|
GFP(S65C)-FLAG-His6
|
3'
|
nmt1**
|
ura4+
|
ura4+, leu1-
|
|
| pDUAL2-CFH61 |
pDUAL2
|
CFP-FLAG-His6
|
3'
|
ef1a-c
|
ura4+
|
ura4+, leu1-
|
|
|
|
* Medium strength nmt1 promoter, ** Low strength nmt1 promoter
|
 |
 |
|
