LOCUS 8703 bp ds-DNA circular 06-MAY-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 1879..2253 /ApEinfo_label=leu1∆5' /ApEinfo_fwdcolor=#804000 /ApEinfo_revcolor=#804000 CDS 847..1563 /ApEinfo_label=CFP /ApEinfo_fwdcolor=#66ccff /ApEinfo_revcolor=#66ccff terminator 1636..1872 /ApEinfo_label=ADH1 terminator /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 CDS 1570..1593 /ApEinfo_label=FLAG epitope(C2) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 gene 6995..8703 /ApEinfo_label=ura4+ /ApEinfo_fwdcolor=#ffcc66 /ApEinfo_revcolor=#ffcc66 CDS 1594..1611 /ApEinfo_label=C-terminal hexahistidine tag /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 rep_origin complement(2268..3460) /ApEinfo_label=ars1 /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=#8000ff gene 3475..3828 /ApEinfo_label=leu1-3' /ApEinfo_fwdcolor=#808000 /ApEinfo_revcolor=#808000 CDS complement(7382..8176) /ApEinfo_label=ura4 ORF∆EcoRV /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 primer_bind complement(3841..3858) /ApEinfo_label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 6917..6937 /ApEinfo_label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 5867..6549 /ApEinfo_label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 promoter 13..815 /ApEinfo_label=ef1a-c promoter /ApEinfo_fwdcolor=#ff6fcf /ApEinfo_revcolor=#ff6fcf rep_origin 4070..4525 /ApEinfo_label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 7..12 /ApEinfo_label=pUC119 /ApEinfo_fwdcolor=#b3b3b3 /ApEinfo_revcolor=#b3b3b3 CDS 3929..3997 /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue misc_binding 6889..6911 /ApEinfo_label=LacO /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue CDS 5110..5769 /ApEinfo_label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow ORIGIN 1 AAGCTTGCAT GCATTTCAAT CATTTTAGGA GACCCTTCTC CGCCTTCACT CATACCTCAT 61 TAGCTGCACG AGTTTTCATG CGCACATATA CATTTGAGTC CTCTCTACCT CCGACTTGAA 121 TGATTTTTAA AGAAACGTTG AAATAAGGGT CAACAGATTT AATCGAGGAG ATCTGCTCCC 181 GAAGTTCTTC TCGAACTTTG CTAATTCAAC TGTTAGACAA AACTATATAT ACACGATAAA 241 AAAGACATAC CGGGCCAAAG AAGTACCCTC CAAAAGCAAA GCCATCAAAA CTATTACCGT 301 TGTGGTGACG GACAAAGAAC AAACAAAAGC AGCTAAGGTG GGTATATCAG CGAAAATCTC 361 AGTCCACAAT CTCAATCCGA TCTGATAACG GCCATCAAAA ATGGTTCAAT GATACAACTA 421 AATATTTTTC ATCATTTAAT AAACCAGATA TATATTTTTA AATTAACGAA AAACGATCCA 481 TTTCATATAT ACAGCGTTGA TCGTTTCCGC CCAATAAATC AAAATCGCCA AGAACGACGT 541 TCGCTAAGCT AATGTTTATT GCGGTGGCAA ATTCGGCTAA GTGTACTCTA AAGACAAAAG 601 GTTAAGAAGC AAAGAAAAAT TTTAAATGAC ATCATGACTT TCCTGCGCAA ACAAAATTCG 661 TTCGCTGATA GGCTCACGAC AATATTTTAA GCCGGAAAAA AAGAAAAAAA CTTTCGAGAC 721 GTAACGAACG TTATGTGACT GCTTTTTTTG CGCAAGTATA TATGTAGGCG CTGGCGCCCT 781 TCGCTTCTAC CTAGAGTCTG TTTCATCGAA CTCACGCTAG CGTCGACAGG CCTGGATCCG 841 GATATCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG 901 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC 961 ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG 1021 CCCACCCTCG TGACCACCCT GACCTGGGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC 1081 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC 1141 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC 1201 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG 1261 GGGCACAAGC TGGAGTACAA CTACATCAGC CACAACGTCT ATATCACCGC CGACAAGCAG 1321 AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG 1381 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC 1441 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC 1501 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC 1561 AAGCCCATGG ACTACAAGGA CGACGATGAT AAACATCACC ACCATCACCA CTGAGATCTC 1621 GACTCTAGAG GATCCGGCGC GCCACTTCTA AATAAGCGAA TTTCTTATGA TTTATGATTT 1681 TTATTATTAA ATAAGTTATA AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG 1741 TTTTAAAACG AAAATTCTTA TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC 1801 AGGTATAGTA TGAGGTCGCT CTTATTGACC ACACCTCTAC CGGCAGATCC GCTAGGGATA 1861 ACAGGGTAAT ATACTAGTGC CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT 1921 GTGAAGGCTT GTTTGGAAGC TGACGGTGTT CTTTTGGGTG CCGTTGGTGG TCCTGAATGG 1981 ACCAACCCCA ATTGTCGTCC TGAGCAAGGT TTATTGAAGC TTCGTAAGAG TATGGGTGTT 2041 TGGGCCAACC TTCGACCTTG CAACTTTGCC AGCAAGTCTT TAGTCAAGTA CAGCCCTTTG 2101 AAGCCTGAAA TCGTTGAAGG TGTCGATTTT TGTGTTGTAC GAGAACTTAC TGGAGGTTGT 2161 TACTTTGGTG AGCGCACTGA GGACAACGGA TCGGGTTATG CTATGGACAC TTGGCCTTAC 2221 AGTTTGGAAG AAGTTTCTCG TATTGCTCGT TTGGCGGCCG CGGGCCCAAC CTTCCAATTC 2281 ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA 2341 GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC 2401 TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG 2461 ATTATTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG 2521 GGGTATGGTT AGTAGCGGCA GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA 2581 GCGAGACAGA ATGGGATACA AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA 2641 TTTGTATTTT TTGTAATTTT ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA 2701 AAACTTCAAC GAAATGTCAA AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC 2761 AGCAACTTAT TCTCCGTTGT AGATTCTTTT TCTTCATAAA TTCCAACAAT GTAACTGCCA 2821 ATTGATTTTT CTCTAGATGC TAATAAATTG AAACTAATGA ATATAATAAC ACAGGTTTTG 2881 ATAAATTGTA AACTCTTACT CAAAAAAAGA ATGTTAAAAC TAATTTAACC TTTTTATAAA 2941 GGCTCATGGC GTTAAAGAGG ATAGTAAAAA TTCTACAATT TTTTAATTTA ATGTCACAAT 3001 TGTCTTGCGA ATATAATTCT ATTAATTTTC GCTAACTGCT GTTTTAGTAC TATTACTTTC 3061 AATATGCCTT ATATGCAACT TTAATTCATG AAGAATGCAA TATTTTTGCA ATCTTTCTTG 3121 TACTGAGCGC TTTTTATCAT TTAGTACTCA TTATTTAATT TTTCAGTAAA GGGCAATAAA 3181 TAAATTTGTA GAAGATGCAA TGTAATCTCC TCTATCCTTT TGCTCATATA TGTTTATGAG 3241 TATACCTAGT CTAGAAAGGC TTGTATTTAA AATATTATTC AATAAAAATT CACAATTTTT 3301 ACGACATGTG CTACCATTCA CTTAACTTCC TGATTATAAA ATTGGTTCGT TTATACTAAT 3361 TACTTAAGTA CCTTTAACTA AACAAAATGC CTATATATAT ATTAATTTAC AATGAGTGTC 3421 AGATAAGTCA CTATGTCCGA GTGGTTAAGG AGTTAGACTC GGGCCCGCGG CCGCTTAGCT 3481 GAAACTTCCA ACCCTCCTGC TCCCGTCACA TTACTCGACA AAGCTAATGT TTTGGCAACT 3541 TCTCGTCTTT GGCGCAAGAC TGTAGCTAAG ATTTTTAAGG AGGAATATCC TCACCTTACC 3601 TTAAAAAACC AGCTCATTGA CTCCGCAGCC ATGCTTTTGG TCAAGAGCCC TCGTACACTT 3661 AACGGTGTTG TTTTGACTGA CAACTTGTTT GGTGACATTA TTTCAGATGA GGCTTCTGTC 3721 ATTCCTGGTA GCTTGGGCCT TTTGCCTTCT GCCTCCCTTT CCGGTGTGGT AGGAAAATCA 3781 GAAGAAAAGG TTCATTGTTT GGTTGAGCCC ATTCACGGTA GCGCTCCCGA GCTCGAATTC 3841 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG 3901 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG 3961 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT 4021 TACGCATCTG TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT 4081 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 4141 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 4201 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 4261 CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 4321 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 4381 CAAACTGGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG 4441 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 4501 AACAAAATAT TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC 4561 GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT 4621 CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG 4681 AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT 4741 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA 4801 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 4861 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT 4921 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 4981 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 5041 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT 5101 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC 5161 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC 5221 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 5281 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 5341 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG 5401 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA 5461 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 5521 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 5581 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 5641 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG 5701 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT 5761 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 5821 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC 5881 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 5941 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 6001 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 6061 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 6121 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 6181 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 6241 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 6301 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA 6361 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 6421 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 6481 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 6541 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 6601 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT 6661 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA 6721 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG 6781 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 6841 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC 6901 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGTGATATTG 6961 ACGAAACTTT TTGACATCTA ATTTATTCTG TTCCAACACC AATGTTTATA ACCAAGTTTT 7021 ATCTTGTTTG TCTACATGGT ATTTTACATT CATCTACATA CATCTTTCAT TGGCTTTGTA 7081 CATAGTTATC ATTACAAGTC TAAAAAAATT CACTCTTTTC TTATTCAATG TCAATCCAAG 7141 AGAAAAGATT GTGGTAATGT TGTAGGAGCA TGTTTAATAA ATTACTATAG CAAATTACTT 7201 TTTATTCCCA AGGTGTTTAT CTATAATAGT TAATATTTTA GTCGCTACAT AAAATTTTAC 7261 CAAAGAGTAC TTGTATACTA ATTCTAAATG CCTTCTGACA TAAAACGCCT AGGAAAACAA 7321 ACGCAAACAA GGCATCGACT TTTTCAATAA CCAACCAAAA AAATTTTACA TTAGTCTTTT 7381 TTTAATGCTG AGAAAGTCTT TGCTGATATG CCTTCCAACC AGCTTCTCTA TATCTCTTGG 7441 CTTCGACAAC AGGATTACGA CCAGCTCCAT AGACTCCACG ACCAACAATG ATGATGTCGC 7501 TACCGCAGTT TACAATCACT TCTTCAGGAG TACGATATTG CTGTCCCAGC CCGTCTCCTT 7561 TAACATCCAA GCCGATACCA GGGGACATAG TTATGTAGTC GCTTTGAAGG TTAGGAAATC 7621 GACGACCAGC TATAAAGCCA AAGCAAAAAT CGGTATGCTT CTCAAACCAT TCTAAGGTTT 7681 TCTCTGTGTA GGAACCAGTA GCCAAAGAGC CTTTGGAAGA CATTTCAGCC AAAAGCAAGA 7741 GACCACGTCC CAAAGGTAAA CCAACTTCTT TGAGGCCTTG TATAATACCC TCGCCTGGCA 7801 CTGTATGGCA ATTTGTGATA TGAGCCCAAG AAGCAATTTT GTACACACCA GATGCATATT 7861 GTAGCTTGAC GGTATTTCCA ATGTCTGCGA ATTTGCGATC CTCAAAGATA AGAAAACGAT 7921 GCTTTTTACC TAAGGCCACC AGTTTTTCTA CCATATCCTG GTCGAAATCC TCGACAACGT 7981 CAATATGTGT CTTGATAACA CAGACATAGG GTCCAATTTT ATCTACCAAT TCTAAGATTT 8041 CGGATTTCTT CGTCAAATCG ACCGCGACTG ACAAGTTGCT TTGCTTTTCT TCCATCAAAG 8101 CCAACAATTC CTTGGCAATG GGATTTTTCA TCCCCTCAGC TCTAGCTGAA TAGCTTTGAA 8161 ATACTCTAGC ATCCATAACT TTGCTTTTAA ACCTTTAATT TCGATCCAAG CAAAAAAGAG 8221 GTTCTTGGTA GGACAATACG GTAAGAAAAC ACGACATGTG CAGAGATGCC GACGAAGCAT 8281 AGTTAAACTG GGATGGTAAA ATCAATTAAG AATTTATAAA GACAAAATTG TATAAGTCTC 8341 TAAAACATCT TAATTATACC TCACAGAACT ATCTAAAATA TATTCACAAA GTGCAAACAT 8401 TATCATGAAA AAGAACCATT TTAATTTAAA GCAAGGGCAT TAAGGCTTAT TTACAGAATT 8461 TCTTACTTTT GTAAAGATTA TAAGGCTGAT TATCTTTTTC ACCATGCCAA AAATTACACA 8521 AGATAGAATG GATGTTTGAA ATTAAACGTG AGTATACAAA CAAATACACT AGGTAAATCG 8581 AAACATTTTT TTCTCCATTA AGTAACAAAT TCCTATTTAG AGAAAGAATG CTGAGTAGAT 8641 TAAATAATCT ATACAAACTT TTTTAACACA AATGCATACA TATAGCCAGT GGGATTTGTA 8701 GCT //