| Gene name |
SPCC1223.15c |
| Gene ID |
52/E04 |
| Gene synonyms/obsolete |
spc19 |
| Gene product |
DASH complex
subunit |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
548 |
| ORF length (spliced) |
447 |
| Entry clone length |
548 |
| No. of intron |
2 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1223.15c.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTATTTGGATGGGTA |
| Rev primer name |
SPCC1223.15c.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGACCTCGTTTTTGTTG |
| Amino acid length |
148 |
| Molecular weight |
17 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
|
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
|
| Localization (YFP) |
Not determined |
| Comments for localization |
|
| Effect of LMB on protein
localization |
Not determined |
| Microscope used for
observation |
|