Gene name |
SPAC56F8.08 |
Gene ID |
52/A12 |
Gene synonyms/obsolete |
ddi1 |
Gene product |
UBA domain;
ubiquitin-binding protein; involved in protein
deubiquitination; SNARE complex binding protein |
Entry clone |
Cloned directly into
pDUAL-FFH1 |
ORF length (unspliced) |
1113 |
ORF length (spliced) |
999 |
Entry clone length |
1113 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC56F8.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAATTTAACACCAGA |
Rev primer name |
SPAC56F8.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGGAGAAAAGAAGCAGCT |
Amino acid length |
332 |
Molecular weight |
35.8 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAAALFVDLPI |
Localization (YFP) |
no expression
clone |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|