| Gene name |
SPBPB8B6.02c |
| Gene ID |
51/A04 |
| Gene synonyms/obsolete |
SPAP8B6.02c;
SPAPB8B6.02c |
| Gene product |
urea transporter;
similar to Sp SPAC869.03C (paralog); similar to Sp
SPBC23G7.13C (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2022 |
| ORF length (spliced) |
|
| Entry clone length |
2022 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
439C:T / 587T:C /
856T:C / 932T:C / 1064T:C / 1588G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAP8B6.02.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGAGCCTATCTTAAACCA |
| Rev primer name |
SPAP8B6.02.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGGCAGTTTTAAGTTCAGAT |
| Amino acid length |
673 |
| Molecular weight |
72.6 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
14 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |