Gene name |
SPAPB1E7.03 |
Gene ID |
49/C10 |
Gene synonyms/obsolete |
|
Gene product |
DNA directed RNA
polymerase III (C74 subunit) |
Entry clone |
Cloned# |
ORF length (unspliced) |
1855 |
ORF length (spliced) |
1776 |
Entry clone length |
1855 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAPB1E7.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCACAGTATGCTGTAGA |
Rev primer name |
SPAPB1E7.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAATTATCCCTAAATACT |
Amino acid length |
591 |
Molecular weight |
68.3 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB?; nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |