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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB2B2.06c
Gene ID 49/C08
Gene synonyms/obsolete
Gene product putative 5' nucleotidase family protein; calcineurin-like phosphodiesterase; similar to Sp SPAC1039.02 and SPAC17G6.03
Entry clone Cloned
ORF length (unspliced) 1806
ORF length (spliced)
Entry clone length 1806
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBPB2B2.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGACAGCCTCGATACA
Rev primer name SPBPB2B2.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATCACAATTATCACTCCAT
Amino acid length 601
Molecular weight 68.4
Isoelectric point (calc.) 4.6
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization except nucleolus
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
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