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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1399.05c
Gene ID 49/B03
Gene synonyms/obsolete
Gene product putative involvement in maltose fermentation zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain; involved in transcriptional regulation
Entry clone Cloned
ORF length (unspliced) 1707
ORF length (spliced) 1590
Entry clone length 1707
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1399.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCGAAGTGGAACGGAC
Rev primer name SPAC1399.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGCCACGTTCCACAGTAGCG
Amino acid length 529
Molecular weight 60.5
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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