| Gene name |
SPAC23E2.02 |
| Gene ID |
48/F11 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
similar to Sp SPBC146.09C; amine oxidase; HMG box; SWIRM
domain; predicted chromosomal protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4040 |
| ORF length (spliced) |
3822 |
| Entry clone length |
4040 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC23E2.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCTCGGGCGTTCTTC |
| Rev primer name |
SPAC23E2.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATATCGTGTTCCATTATAA |
| Amino acid length |
1273 |
| Molecular weight |
142.4 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLNLLNKQLGI/LSYNLGRLHI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
too dark to
photo |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |