| Gene name |
SPAC17H9.10c |
| Gene ID |
48/A11 |
| Gene synonyms/obsolete |
ddb1 |
| Gene product |
UV damage binding
factor; XP-E family; involved in DNA replication checkpoint;
no apparent Sc ortholog; non-essential; deletion results in
elongated cells; deletion results in nuclear morphology
defects; deletion sensitive to DNA damaging agents |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3219 |
| ORF length (spliced) |
|
| Entry clone length |
3219 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17H9.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTATGTTACTTATTT |
| Rev primer name |
SPAC17H9.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGATGTAGTTTCTCAAGA |
| Amino acid length |
1072 |
| Molecular weight |
120.6 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCATVDGSLMI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |