Gene name |
SPAC20G8.09c |
Gene ID |
48/A10 |
Gene synonyms/obsolete |
|
Gene product |
conserved protein;
acetyltransferase; Sc KRE33 is Killer toxin resistant; Sc
KRE33 is null lethal; Sc KRE33 is induced by glucose
repression |
Entry clone |
Cloned |
ORF length (unspliced) |
3209 |
ORF length (spliced) |
3102 |
Entry clone length |
3209 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC20G8.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCAAGAAAGCACTTGA |
Rev primer name |
SPAC20G8.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTCCTAAATTTCTTCTTG |
Amino acid length |
1033 |
Molecular weight |
116.4 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |