Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC694.06c
Gene ID 47/G10
Gene synonyms/obsolete mrc1
Gene product involved in DNA replication checkpoint; involved in the activation of Cds1p during replication stress (required); involved in chromatin silencing; predicted coiled-coil; DEK-rich protein; similar to human Claspin
Entry clone Cloned
ORF length (unspliced) 3060
ORF length (spliced)
Entry clone length 3060
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC694.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATCTTTAGACGAGAA
Rev primer name SPAC694.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCAAAGTCCGAGTAATTA
Amino acid length 1019
Molecular weight 114.3
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.