Gene name |
SPAC20G8.08c |
Gene ID |
47/F12 |
Gene synonyms/obsolete |
|
Gene product |
SNF2 family; helicase
C-terminal domain; DEAD/DEAH box helicase; similar to Sp
SPCC1235.05C and SPAC25A8.01C |
Entry clone |
Cloned |
ORF length (unspliced) |
3001 |
ORF length (spliced) |
2835 |
Entry clone length |
3001 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC20G8.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAGAAAAGATAATAA |
Rev primer name |
SPAC20G8.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAACGGACCCATCCATA |
Amino acid length |
944 |
Molecular weight |
108.2 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLYLSVLEL/LEYVLNTLDL |
Localization (YFP) |
nucleus; nuclear dots
|
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |