Gene name |
SPBC30B4.05 |
Gene ID |
47/E12 |
Gene synonyms/obsolete |
|
Gene product |
karyopherin-beta;
involved in nuclear export; interacts with centromeric element
CDEII; involved in chromosome segregation |
Entry clone |
Cloned |
ORF length (unspliced) |
2953 |
ORF length (spliced) |
2904 |
Entry clone length |
2953 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC30B4.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGACATTCCAACTCT |
Rev primer name |
SPBC30B4.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGATCACGTAACCATATGAT |
Amino acid length |
967 |
Molecular weight |
109.4 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPALNQLFL/LDRKISALGL/LRLLTCDLVL |
Localization (YFP) |
nuclear envelope |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |