Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC613.13c
Gene ID 47/D05
Gene synonyms/obsolete rhp16; rad16; SPCC330.01c
Gene product involved in DNA repair; involved in nucleotide-excision repair; zinc finger protein; zf-C3HC4 type (RING finger); ubiquitin ligase (E3); SNF2 familyj; DEAD/DEAH box helicase; helicase C-terminal domain
Entry clone Cloned
ORF length (unspliced) 2892
ORF length (spliced)
Entry clone length 2892
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC613.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCATATATCTACTCTAAA
Rev primer name SPCC613.13.Rv
Rev primer SEQ AGAAAGCTGGGTAATTTGAGAACAAGAATTGC
Amino acid length 963
Molecular weight 109.3
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 288
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.