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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC637.14
Gene ID 47/C05
Gene synonyms/obsolete SPAC31F12.01
Gene product zds family; involved in cell cycle
Entry clone Cloned
ORF length (unspliced) 2817
ORF length (spliced)
Entry clone length 2817
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC637.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTAGTTCTTCAGTTTC
Rev primer name SPAC637.14.Rv
Rev primer SEQ AGAAAGCTGGGTATTTAGCATTCTTTGAAACT
Amino acid length 938
Molecular weight 103
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSNFMYSYLDL
Localization (YFP) periphery at cell tip and site of septum formation; cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica; DeltaVision

Image information
YFP 4 images) See all images

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