Gene name |
SPCC1672.06c |
Gene ID |
47/B11 |
Gene synonyms/obsolete |
asp1 |
Gene product |
acid phosphatase;
important for the function of the cortical actin cytoskeleton;
deletion mutant results in altered morphology and growth
properties at elevated temperatures; deletion mutant results
in polarized growth defects; non-essential; synthetically
lethal with mutations in Arp2/3 complex components |
Entry clone |
Cloned |
ORF length (unspliced) |
2763 |
ORF length (spliced) |
|
Entry clone length |
2763 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1672.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTCAAAATGCAAGTCA |
Rev primer name |
SPCC1672.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTAATGTTAACAGGAATA |
Amino acid length |
920 |
Molecular weight |
105.6 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGEQFHKDLYI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |