| Gene name |
SPBC19C7.11 |
| Gene ID |
46/H06 |
| Gene synonyms/obsolete |
|
| Gene product |
ClC chloride channel
predicted; similar to S. cerevisiae YJR040W |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2439 |
| ORF length (spliced) |
|
| Entry clone length |
2439 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1126T:deletion |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC19C7.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTCTCAAAAGAGAGC |
| Rev primer name |
SPBC19C7.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTATGTTTAATTTTAAG |
| Amino acid length |
812 |
| Molecular weight |
90.5 |
| Isoelectric point (calc.) |
7.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
11 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVKCVGLPLAI/LLFFCFLGI/LVSFLNPWLRL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |