Gene name |
SPBC19C7.11 |
Gene ID |
46/H06 |
Gene synonyms/obsolete |
|
Gene product |
ClC chloride channel
predicted; similar to S. cerevisiae YJR040W |
Entry clone |
Cloned |
ORF length (unspliced) |
2439 |
ORF length (spliced) |
|
Entry clone length |
2439 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1126T:deletion |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC19C7.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTCTCAAAAGAGAGC |
Rev primer name |
SPBC19C7.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCTATGTTTAATTTTAAG |
Amino acid length |
812 |
Molecular weight |
90.5 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
11 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVKCVGLPLAI/LLFFCFLGI/LVSFLNPWLRL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |