Gene name |
SPCC965.04c |
Gene ID |
46/G05 |
Gene synonyms/obsolete |
|
Gene product |
AAA family ATPase;
metallopeptidase |
Entry clone |
Cloned |
ORF length (unspliced) |
2231 |
ORF length (spliced) |
2130 |
Entry clone length |
2231 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
1818T:G |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC965.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAGGGTTTTGCATCC |
Rev primer name |
SPCC965.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACTTAGCTTATTACGAAGT |
Amino acid length |
709 |
Molecular weight |
78.2 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPDVRGRLAI |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |