Gene name |
SPBC365.10 |
Gene ID |
46/G02 |
Gene synonyms/obsolete |
|
Gene product |
actin-like protein;
involved in intracellular protein transport; involved in
protein-vacuolar targeting |
Entry clone |
Cloned |
ORF length (unspliced) |
2221 |
ORF length (spliced) |
2166 |
Entry clone length |
2221 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC365.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAATATATGCTGTTAG |
Rev primer name |
SPBC365.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGAATTTATGTTTCCTAAA |
Amino acid length |
721 |
Molecular weight |
82.3 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDYTFLKLGI |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
cytoplasmic aggregates
by over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |