Gene name |
SPAC105.03c |
Gene ID |
46/E11 |
Gene synonyms/obsolete |
|
Gene product |
putative
transcriptional activator; possible activator for allantoin,
4-aminobutyric acid (GABA), and urea catabolic genes; zinc
finger protein (201others); zf-fungal Zn(2)-Cys(6) binuclear
cluster domain; similar to Sp SPAC25B8.11 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
2127 |
ORF length (spliced) |
|
Entry clone length |
2127 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC105.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACCAAAGTTCATCTCC |
Rev primer name |
SPAC105.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTCAGGGTTTCGTCCAAG |
Amino acid length |
708 |
Molecular weight |
80.9 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFLQIDALEL |
Localization (YFP) |
periphery;
vacuole |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |