Gene name |
SPAC23D3.10c |
Gene ID |
46/E09 |
Gene synonyms/obsolete |
eng2 |
Gene product |
glycosyl hydrolase
family 81; endo-1,3-beta-glucanase; similar to Sp eng1 |
Entry clone |
Cloned |
ORF length (unspliced) |
2121 |
ORF length (spliced) |
|
Entry clone length |
2121 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC23D3.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGTTTTAGTACCAAT |
Rev primer name |
SPAC23D3.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGTGATCCACCCATACCA |
Amino acid length |
706 |
Molecular weight |
77.9 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPTQVGFLPI |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |