Gene name |
SPAC1834.07 |
Gene ID |
46/D06 |
Gene synonyms/obsolete |
krp1; klp3 |
Gene product |
kinesin-like protein;
affects golgi membrane recycling; overexpression inhibits
mitotic growth; overexpression results in aberrant septa;
interacts physically with Kap1p; involved in cytokinesis |
Entry clone |
Cloned |
ORF length (unspliced) |
2044 |
ORF length (spliced) |
1665 |
Entry clone length |
2044 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1834.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTCAATTAAAGTAGT |
Rev primer name |
SPAC1834.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTGCCCCCTTGAATTTTC |
Amino acid length |
554 |
Molecular weight |
61.9 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
large bright dots;
small cytoplasmic dots at periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |