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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F3.09
Gene ID 46/C08
Gene synonyms/obsolete
Gene product hypothetical protein; conserved protein
Entry clone Cloned
ORF length (unspliced) 2023
ORF length (spliced) 1686
Entry clone length 2023
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1F3.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTTTTTATGACAAACT
Rev primer name SPAC1F3.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGTAAAGTCGTAAGGTTTG
Amino acid length 561
Molecular weight 63.2
Isoelectric point (calc.) 8.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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