Gene name |
SPBP8B7.23 |
Gene ID |
46/C07 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3) |
Entry clone |
Cloned |
ORF length (unspliced) |
2022 |
ORF length (spliced) |
|
Entry clone length |
2022 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBP8B7.23.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTAACGGAGAAAAATC |
Rev primer name |
SPBP8B7.23.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCGATGGGCGGCACCTGTT |
Amino acid length |
673 |
Molecular weight |
76.4 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQTDIDNLCL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
Leica |