| Gene name |
SPAC7D4.10 |
| Gene ID |
46/B03 |
| Gene synonyms/obsolete |
vma13 |
| Gene product |
vacuolar ATP synthase
(subunit H); V-type ATPase (H subunit); vacuolar proton pump
component; V1 sector; di-leucine-binding domain possibly
consists of a HEAT or ARM repeat protein fold |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1901 |
| ORF length (spliced) |
1353 |
| Entry clone length |
1901 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC7D4.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAATAGTGATTTAGA |
| Rev primer name |
SPAC7D4.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAAGTAATTTTACTTAGG |
| Amino acid length |
450 |
| Molecular weight |
51.4 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |