Gene name |
SPBC25D12.02c |
Gene ID |
46/A05 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan; serine-rich protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1852 |
ORF length (spliced) |
1800 |
Entry clone length |
1852 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC25D12.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAACTACATTAAGGCT |
Rev primer name |
SPBC25D12.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACACTAGGGCCGCCAAGCCA |
Amino acid length |
599 |
Molecular weight |
65.9 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
bright dots by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |