Gene name |
SPAC1B1.02c |
Gene ID |
45/D04 |
Gene synonyms/obsolete |
|
Gene product |
NAD kinase; ATP
dependent; involved in nicotinate and nicotinamide metabolism;
similar to Sp SPAC3H5.11 and SPCC24B10.02C |
Entry clone |
Cloned |
ORF length (unspliced) |
1614 |
ORF length (spliced) |
|
Entry clone length |
1614 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1B1.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACCGGGCTCGAAACT |
Rev primer name |
SPAC1B1.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTTTGCTCTTCGGAAGTA |
Amino acid length |
537 |
Molecular weight |
60.7 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTKKLSSLSI/LAFRFRKVLLI |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
Microscope used for
observation |
Leica |