| Gene name |
SPAC694.04c |
| Gene ID |
44/C05 |
| Gene synonyms/obsolete |
|
| Gene product |
metal dependent
hydrolase; citidyltransferase; phosphatidylcholine
biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1318 |
| ORF length (spliced) |
975 |
| Entry clone length |
1318 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC694.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAATTTAGTTAAAAT |
| Rev primer name |
SPAC694.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTGTGGAAAATCTAAA |
| Amino acid length |
324 |
| Molecular weight |
36.6 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFDIEKELGI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |