| Gene name |
SPBC18H10.08c |
| Gene ID |
44/C04 |
| Gene synonyms/obsolete |
ubp4 |
| Gene product |
ubiquitin C-terminal
hydrolase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1317 |
| ORF length (spliced) |
|
| Entry clone length |
1317 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
710C:T |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC18H10.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTCTTCAAAAATATA |
| Rev primer name |
SPBC18H10.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTAACATTTCTTTTCAAA |
| Amino acid length |
438 |
| Molecular weight |
49.7 |
| Isoelectric point (calc.) |
7.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYSMLRSLKL |
| Localization (YFP) |
bright cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |