| Gene name |
SPAC521.02 |
| Gene ID |
43/E02 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc
metallopeptidase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
852 |
| ORF length (spliced) |
|
| Entry clone length |
852 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC521.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCGCTATATTGTATTT |
| Rev primer name |
SPAC521.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTACTACGAAATAGTTT |
| Amino acid length |
283 |
| Molecular weight |
32.3 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
198/196 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSVSFMLRI/LGTDMNIRLEL |
| Localization (YFP) |
nucleus; ambiguous
structure |
| Comments for localization |
ER? Septum? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |