| Gene name |
SPBC25D12.07c |
| Gene ID |
43/D09 |
| Gene synonyms/obsolete |
SPBC13G1.14c |
| Gene product |
RNA-binding protein;
involved in mRNA splicing; rrm RNA recognition motif; no
apparent Sc ortholog; similar to human Rnps1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
845 |
| ORF length (spliced) |
732 |
| Entry clone length |
845 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC25D12.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGATTTCCACTTTTGT |
| Rev primer name |
SPBC25D12.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGACGATGTCTATAGGAG |
| Amino acid length |
243 |
| Molecular weight |
28.3 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |