Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1442.15c
Gene ID 42/D09
Gene synonyms/obsolete
Gene product putative cytochrome C oxidase biogenesis; no apparent Sc ortholog; related to Sp oxa101 and oxa102
Entry clone Cloned
ORF length (unspliced) 609
ORF length (spliced)
Entry clone length 609
No. of intron 0
Sequence status Finished
Sequence results 601C:A
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC1442.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTCAAACATTAATAGG
Rev primer name SPCC1442.15.Rv
Rev primer SEQ AGAAAGCTGGGTATAACAGCTTTCTCAATGCA
Amino acid length 202
Molecular weight 23
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, Zeiss

Image information
YFP 4 images) See all images

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