Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC11B10.08
Gene ID 41/D09
Gene synonyms/obsolete pi003; SPACTOKYO_453.33c
Gene product conserved hypothetical; hypothetical protein; WW domain
Entry clone Cloned
ORF length (unspliced) 615
ORF length (spliced)
Entry clone length 615
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC11B10.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTATCAAACTCGTGA
Rev primer name SPBC11B10.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGAAATCATTGCCAAAG
Amino acid length 204
Molecular weight 22
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus>>cytosol
Comments for localization bright nuclear dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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