Gene name |
SPBC11B10.08 |
Gene ID |
41/D09 |
Gene synonyms/obsolete |
pi003;
SPACTOKYO_453.33c |
Gene product |
conserved
hypothetical; hypothetical protein; WW domain |
Entry clone |
Cloned |
ORF length (unspliced) |
615 |
ORF length (spliced) |
|
Entry clone length |
615 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC11B10.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTATCAAACTCGTGA |
Rev primer name |
SPBC11B10.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGAAATCATTGCCAAAG |
Amino acid length |
204 |
Molecular weight |
22 |
Isoelectric point (calc.) |
4.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nuclear dots;
nucleus>>cytosol |
Comments for localization |
bright nuclear dots
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |