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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB2B2.15
Gene ID 41/D08
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein; similar to Sp SPAC977.05C (paralog)
Entry clone Cloned
ORF length (unspliced) 612
ORF length (spliced)
Entry clone length 612
No. of intron 0
Sequence status Finished
Sequence results 253A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBPB2B2.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTCAAAAACTTAATATT
Rev primer name SPBPB2B2.15.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTGAATCCCTCATCCAAA
Amino acid length 203
Molecular weight 22.6
Isoelectric point (calc.) 4.3
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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