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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC15D4.01c
Gene ID 40/G11
Gene synonyms/obsolete klp1; SPBC2D10.21c
Gene product kinesin-like protein
Entry clone Cloned
ORF length (unspliced) 2075
ORF length (spliced) 1902
Entry clone length 2075
No. of intron 2
Sequence status Finished
Sequence results 1257G:A / 1617T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC15D4.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATACAGGTAAGCAATTT
Rev primer name SPBC15D4.01.Rv
Rev primer SEQ AGAAAGCTGGGTACAATTCATTAATATCGATA
Amino acid length 633
Molecular weight 71.4
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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