| Gene name |
SPBC16G5.18 |
| Gene ID |
40/D08 |
| Gene synonyms/obsolete |
erg24 |
| Gene product |
sterol c-14 reductase;
involved in ergosterol biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1275 |
| ORF length (spliced) |
|
| Entry clone length |
1275 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
212T:C / 711A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16G5.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTAAAGGTGCGGTAAA |
| Rev primer name |
SPBC16G5.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATACACATAGGGAATAATA |
| Amino acid length |
424 |
| Molecular weight |
48.5 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLWFSTLTL/LGLVKTLAI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |