Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC543.05c
Gene ID 39/F01
Gene synonyms/obsolete
Gene product anion exchange family protein inorganic anion exchanger; H303-transporter family
Entry clone Cloned
ORF length (unspliced) 1769
ORF length (spliced) 1554
Entry clone length 1769
No. of intron 4
Sequence status Finished
Sequence results 155T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC543.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAAAAAGGATAAGAA
Rev primer name SPBC543.05.Rv
Rev primer SEQ AGAAAGCTGGGTATACTTTTTGATAAGCAATA
Amino acid length 517
Molecular weight 58.4
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSITIALLFL/LAIPFSIVLTI
Localization (YFP) vacuole
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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