| Gene name |
SPBC30D10.13c |
| Gene ID |
39/E10 |
| Gene synonyms/obsolete |
pdb1 |
| Gene product |
pyruvate dehydrogenase
e1 component beta pyruvate dehydrogenase e1 component (beta
subunit); involved in acetyl-CoA biosynthesis from pyruvate;
pyruvate dehydrogenase (lipoamide) activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1101 |
| ORF length (spliced) |
|
| Entry clone length |
1101 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC30D10.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTCGTCTTCAAAAGTT |
| Rev primer name |
SPBC30D10.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTAATATACAAGCATTTT |
| Amino acid length |
366 |
| Molecular weight |
39.6 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSIRPLDI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregates |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |