Gene name |
SPAC6C3.06c |
Gene ID |
39/B06 |
Gene synonyms/obsolete |
|
Gene product |
putative
cation-transporting ATPase P-type ATPase; P4 type;
aminophospholipid translocase |
Entry clone |
Cloned |
ORF length (unspliced) |
3243 |
ORF length (spliced) |
3102 |
Entry clone length |
3243 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
1046T:C /
2533T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6C3.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACTCAAGACTAAATCG |
Rev primer name |
SPAC6C3.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGTTGCAGCTTTGCATAA |
Amino acid length |
1033 |
Molecular weight |
116.5 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
10 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSQLIPPLKI/LCTFVLVLSI/LVRNLVLALSL/LNNNVYKILNI/LENDMDLLGL/LQKDVKITLEL/LISVYQGLII |
Localization (YFP) |
Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |