| Gene name |
SPCC1672.07 |
| Gene ID |
39/B02 |
| Gene synonyms/obsolete |
|
| Gene product |
beta transducin WD
repeat protein; U3 snoRNP protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2768 |
| ORF length (spliced) |
2709 |
| Entry clone length |
2768 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
408A:G / 794T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1672.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCATTTGCAGAGAAGAA |
| Rev primer name |
SPCC1672.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTACGCATAAAGCTCAAA |
| Amino acid length |
902 |
| Molecular weight |
100.5 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; periphery at
site of septum formation; nucleus>>cytosol; spindle
microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |