| Gene name |
SPBC16E9.11c |
| Gene ID |
39/A04 |
| Gene synonyms/obsolete |
pub3 |
| Gene product |
ubiquitin--protein
ligase ubiquitin-protein ligase (E3); HECT domain; WW domain;
C2 domain; similar to Sp SPAC11G7.02 and SPAC1805.15C |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
2703 |
| ORF length (spliced) |
2361 |
| Entry clone length |
2703 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC16E9.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACAAGGAGCGAAAAG |
| Rev primer name |
SPBC16E9.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCGTTTCCAAACCCTACC |
| Amino acid length |
786 |
| Molecular weight |
89.2 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSVLGNLVL/LENDITGILDL |
| Localization (YFP) |
periphery |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |