Gene name |
SPAC1B1.03c |
Gene ID |
38/E07 |
Gene synonyms/obsolete |
|
Gene product |
karyopherin-beta;
targets proteins with nuclear localization (NLS) sequences to
the nuclear pore complex; armadillo repeat protein; HEAT
repeat |
Entry clone |
Cloned |
ORF length (unspliced) |
2592 |
ORF length (spliced) |
|
Entry clone length |
2592 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
2023A:C/ 1933T:C |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1B1.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACGCAGGTGAGTTTTT |
Rev primer name |
SPAC1B1.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTCTAGCTTGACGCTTA |
Amino acid length |
863 |
Molecular weight |
94.7 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPMIANVLSI |
Localization (YFP) |
nuclear envelope |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |