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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC365.15
Gene ID 38/C08
Gene synonyms/obsolete alp4
Gene product spindle pole body component; gamma tubulin complex; involved in G1 progression (required); involved in spindle assembly checkpoint; involved in cytokinesis
Entry clone Cloned
ORF length (unspliced) 2355
ORF length (spliced)
Entry clone length 2355
No. of intron 0
Sequence status Finished
Sequence results 505A:G / 540T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC365.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTGAGATCGATTAGTTC
Rev primer name SPBC365.15.Rv
Rev primer SEQ AGAAAGCTGGGTATCCCGTCAATTTCATTACT
Amino acid length 784
Molecular weight 90.1
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LCYKVPFPLSL
Localization (YFP) SPB; periphery at site of septum formation; nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 4 images) See all images

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