| Gene name |
SPBC29A3.09c |
| Gene ID |
38/A04 |
| Gene synonyms/obsolete |
|
| Gene product |
non-transporter with
ATP binding cassette; involved in amino acid starvation
response; protein kinase activator |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2211 |
| ORF length (spliced) |
|
| Entry clone length |
2211 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1487A:G /
1982A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC29A3.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGCAAGTCATTCAGAA |
| Rev primer name |
SPBC29A3.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTTTTTATAGTCATGGTT |
| Amino acid length |
736 |
| Molecular weight |
83.7 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus, partially |
| Microscope used for
observation |
Leica |