Gene name |
SPAC6B12.12 |
Gene ID |
36/H11 |
Gene synonyms/obsolete |
|
Gene product |
mitochondrial outer
membrane translocase complex; involved in mitochondrial outer
membrane protein import; TPR repeat protein; mitochondrial
import receptor |
Entry clone |
Cloned |
ORF length (unspliced) |
1878 |
ORF length (spliced) |
|
Entry clone length |
1878 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
536C:T / 605A:G /
1629T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6B12.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGGTATATCCAATGC |
Rev primer name |
SPAC6B12.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATAGGATTGCTTAAACGA |
Amino acid length |
625 |
Molecular weight |
69.5 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
109 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |