| Gene name |
SPBP8B7.14c |
| Gene ID |
36/H09 |
| Gene synonyms/obsolete |
dpb2 |
| Gene product |
DNA polymerase epsilon
(subunit b); essential; involved in DNA replication
(initiation); assembly of the replicative complex; origin
binding |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1867 |
| ORF length (spliced) |
1785 |
| Entry clone length |
1867 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1717T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP8B7.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACAATTCCATTACGGA |
| Rev primer name |
SPBP8B7.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATTCTCTCCTTGTGTGTA |
| Amino acid length |
594 |
| Molecular weight |
67.2 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKALNNLDI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |