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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.14c
Gene ID 36/H09
Gene synonyms/obsolete dpb2
Gene product DNA polymerase epsilon (subunit b); essential; involved in DNA replication (initiation); assembly of the replicative complex; origin binding
Entry clone Cloned
ORF length (unspliced) 1867
ORF length (spliced) 1785
Entry clone length 1867
No. of intron 2
Sequence status Finished
Sequence results 1717T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACAATTCCATTACGGA
Rev primer name SPBP8B7.14.Rv
Rev primer SEQ AGAAAGCTGGGTAAATTCTCTCCTTGTGTGTA
Amino acid length 594
Molecular weight 67.2
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKALNNLDI
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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