Gene name |
SPAC926.06c |
Gene ID |
36/H08 |
Gene synonyms/obsolete |
|
Gene product |
leucine-rich repeat
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1866 |
ORF length (spliced) |
|
Entry clone length |
1866 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC926.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGTCTACTGTCGACGG |
Rev primer name |
SPAC926.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAGATTCATCCTTATTT |
Amino acid length |
621 |
Molecular weight |
69.4 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSTTLKSLIL/LSEVLIKLVL/LQSLVSLDL/LPQLCSLNL/LSHLQILVL |
Localization (YFP) |
cytosol=nucleus;
vacuole membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |