| Gene name |
SPAC19A8.03 |
| Gene ID |
36/C09 |
| Gene synonyms/obsolete |
|
| Gene product |
phosphatidylinositol
3-phosphate phosphatase; similar to protein tyrosine
phosphatases myotubularin-like |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1781 |
| ORF length (spliced) |
1680 |
| Entry clone length |
1781 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1482T:C /
1761T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC19A8.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAACATCAAAGTTGC |
| Rev primer name |
SPAC19A8.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAAGACGGAGATTCGGAG |
| Amino acid length |
559 |
| Molecular weight |
64.1 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCALPQLCL |
| Localization (YFP) |
cytosol; cytoplasmic
dots, especially at cell tip and site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |